Tritium-labeled compounds I. Radioassay of tritium-labeled compounds in ""infinitely thick"" films with a windowless, gas-flow, proportional counter
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منابع مشابه
Tritium - Labeled Compounds III . Aldoses - i - ( , 2
In a n c,," method fo r prepar ing aldoses labeled wit h Lritium at ca r bon l , Lhc aldoni c lactone l S rcdu ced with li thium boro.hyddde-I in p yridine soluLion . The mcthod is suitablc for prcparing aldosesl -1 h a vin g high specifi c act ivit ies. Thc crudc product con tains a substant ia l proport ion of t hc c<?lTespondin g a ldi tol-l-I, but t he purc aldosc-l-1 is rcadil y scparated ...
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A general-purpose manifold is descd bed, which is useful for numerous proced ures involving trit ium gas and tritium-labeled mate rials. M ethods a re given in detail fOI' (a) converting t ritium gas to tritium oxide, (b) preparing tritium-labeled li thium borohy dride, and (c) conducting a variety of r eactions in a closed system. Auxilia ry equipment is shown, including water traps, reaction ...
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By EDWARD ADELSON, RICHARD M. KAUFMAN, Cmo BERDEGUEZ, ARNOLD A. LEAR AND JACK J. RHEINGOLD F OR THE PAST 10 years, P32 labeled diisopropylfluorophosphate has been used to tag platelets.”2 This compound attaches to various proteins, during which process it changes from diisopropylfiuorophosphate ( DFP) to diisopropylphosphate (DIP).3 The DIP is irreversibly bound to the protein molecule until th...
متن کاملPurification of tritium-labeled cholera toxin.
Cholera toxin was labeled with tritium by the Wilzbach technique, and highly purified radiolabeled toxin was obtained by Sephadex column chromatography and disc gel electrophoresis. 3H-labeled cholera toxin retained its biological activity and chemical stability and had a specific activity of 405.9 muCi/mumol. The methods utilized in extraction and purification of 3H-labeled toxin may be advant...
متن کاملA general method of tritium labeling utilizing microwave discharge activation of tritium gas. Methodology and application to biological compounds.
A method of general tritium labeling is described which is applicable to a broad range of organic compounds of biological interest, including amino acids, peptides, and proteins. The method is rapid, inexpensive, and nondestructive, thereby making the purification of labeled material relatively uncomplicated. The reaction apparatus consists of a closed system in which tritium gas at a pressure ...
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ژورنال
عنوان ژورنال: Journal of Research of the National Bureau of Standards Section A: Physics and Chemistry
سال: 1959
ISSN: 0022-4332
DOI: 10.6028/jres.063a.011